2024 Topflash实验方法

Topflash实验方法

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August undefined, 2024

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96515 WebIn our lab, we usually transfect 50ng of DNA per well of a 96 well plate (in which we plate between 10-30K cells, depending cell line). From that 50ng DNA, 15ng are TOPFlash …

【求助】快急死了，再次求助关于wnt通路中TOPflash/FOPflash

WebResearchers who want to detect active Wnt-ßcatenin signaling in cells have various tools available. To measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from various sources including lentivirus-based reporters from the Nusse and ... WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... jonesboro office max

TCF Reporter Plasmid Kit 17-285

Web25. máj 2024 · 载体描述. Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (mutated) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPFLASH (Catalog # 21-170) which contains wild type Tcf binding sites. Web22. sep 2015 · 一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基 … Web25. máj 2024 · TOPFlash载体基本信息订购信息质粒图谱载体描述 The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fate determination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling in Drosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to … how to install date picker in excel

TOPFlash说明书-分析方法-生物在线 Lab-on-Web - BIOON

生物学研究中，什么是TOPflash和FOPflash? - 百度知道

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96420 http://www.biofeng.com/adjy/zhili/M50-Super-8x-TOPFlash.html how to install datylon pluginWebM50 Super 8x TOPFlash载体质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 M50 Super 8x TOPFlash 价格： 1200元联系方式： I47-825O-882O 相关技术服务：质粒构建基因合成质粒大提立刻购买买家导航载体基本信息载体质粒图谱和多克隆位点信息载体简介载体序列 LOCUS M50 Super 8x TOPFlash 4985 bp DNA SYN DEFINITION … jonesboro ophthalmologist

"WebTOPFlash reporter luciferase activities were measured. The relative luciferase activity (fold-change) was reported. * P 0.05 versus controls ( n = 5, t test). ( O – R ) Overexpression of IGF2BP3 triggers the upregulation of KIM-1, p-H3, and p16 INK4A expression in tubular cells. HKC-8 cells were infected with either control lentivirus or ... " - Topflash实验方法

Topflash实验方法

Web1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插入两组TCF/LEF结合位点序列，每组有三个重复序列，一组为正向序列，另一组是它的反向 … Web25. sep 2024 · PLAYER SERVIDOR TOPFLASH P.R History 2.07K subscribers Subscribe 5 960 views 3 years ago OLÁ GALERA HOJE TRAZENDO PARA VOCÊ O NOSSO PLAYER EXCLUSIVO PARA CLIENTES E REVENDEDORES DO...

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http://www.bioon.com.cn/doc/showarticle.asp?newsid=96515 WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : …

WebM50 Super 8x TOPFlash Sequences (3) Addgene Sequences: Full (1) Partial (2) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). ... WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF …

WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : …

WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is activated, beta-catenin will translocate to the nucleus to associate with TCF/LEF transcription factors to activate transcription of Wnt target genes.

WebFOPflash-normalized TOPflash luciferase activity was represented to the relative TCF/LEF luciferase activity. The expression of TNIK and β-catenin in cytosolic and nuclear fractions (B), and the protein levels of TCF4-interacting proteins c were measured by Western blot analysis and immunoprecipitation assay, respectively. Actin, histone H3 ... jonesboro oil changeWebDNA标记和检测> 生物素标记与检测EMSA相关建库与高通量测序 RNA相关> RNA提取RNA电泳切割、合成、连接和修饰RNA保护RNA病毒mRNA疫苗相关无RNA酶试剂Northern与原 … jonesboro orkin reviewsWebTOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild … how to install datto agentWeb21. feb 2012 · 具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。数据处理：通常以TOP/FOP的比值表示。公式如下：转染有TOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧光值F (Top)/采集得到的海肾荧光值R (Top) 本回答被网友采纳 92 评论分享举报 shovel39 2012-02-21 · TA获得超过345个 … jonesboro ofscWeb具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。数据处理：通常以TOP/FOP的比值表示。公式如下：转染有TOP-Flash和 … how to install datetime package in pythonWeb26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … how to install davinci resolveWebtopflash tcf lef luciferase reporter construct - by Bioz Stars , 2024-04 86 / 100 stars Images 1) Product Images from "Small molecule inhibitors of WNT/β-catenin signaling block IL-1β- and TNFα-induced cartilage degradation" Article Title: Small molecule inhibitors of WNT/β-catenin signaling block IL-1β- and TNFα-induced cartilage degradation how to install datto cloud continuity