WebMar 16, 2024 · The digested fragments were ligated with the pET28a and pET26b vectors using T4 ligase (M0202S; Roche, Germany) overnight at 18 °C to insert a hexahistidine (His6)-tag at either the N- or C-terminus of the target protein. Web丁香通为您找到14条dna修复论文造假信息，包括dna修复论文造假报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna修复论文造假,上丁香通。

T4 DNA Ligase NEB

WebSep 9, 2015 · of 0.12 µM [300 µg/ml]) in 20 µl of 1X T4 DNA Ligase Reaction Buffer in 30 minutes at 16°C. Heat Inactivation: 65°C for 10 minutes. T4 DNA Ligase M0202S RECOMBINANT Store at –20°C Lot: 1011202 Description: Catalyzes the formation of a phos-phodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in … WebSep 18, 2024 · Add 1 μL T4 ligase and ∼50 ng of cut vector directly to the reaction from Part Two. b. Allow the ligation to sit at 20°C–22°C (room temperature) for 3 h. c. Remove excess salts via dialysis. d. Transform 1.5 μL of the ligation using 25 μL DH10B electrocompetent E. coli cells. e. After transformation, quickly resuspend in 500 μL LB. f. dinosaurs before time on youtube

Cloning via Restriction Digest McManus Lab

WebThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The … WebFor a standard T4 ligation, mix: 2 μL annealed oligo from step C.2 20 ng digested pLKO.1 TRC-cloning vector from step C.3 (If you were unable to measure the DNA concentration, use 1 μL) 2 μL 10x NEB T4 DNA ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring to 20 μL final volume Incubate at 16°C for 4-20 hours. WebProduct Name: T4 DNA Ligase Catalog Number: M0202S Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ... T4 DNA Ligase is ≥ 95% pure as determined by SDS-PAGE analysis using Coomassie Blue Pass. 240 County Road Ipswich, MA 01938-2723 Tel 978-927-5054 Fax 978-921-1350 … fort sill knox hall