Mcherry gal9
WebCMP05 induced SSO leakage concurrently with endosomal swelling, induced SSO leakage in a mCherry-GAL9-recruitment-dependent manner. CMP05 disrupted normal SSO trafficking through the endosomal network. CMP05 was able to increase ON activity in a more efficacious manner than the previously published compound, UNC2383, in HeLa … WebmCherry (N terminal on backbone) Cloning Information Cloning method Restriction Enzyme 5′ cloning site HindIII (not destroyed) 3′ cloning site EcoRI (not destroyed) 5′ sequencing …
Mcherry gal9
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WebGalectin-9 (GAL9) is a known marker of endosomal membrane disruption and can be utilized to quantitate endosomal rupture 22 . The initially selected 7 compounds and all … WebmCherry-GAL9 PB-GFP-Gal8 pKDsgRNA-p15 pEYFP-C3-HA-Ubiquitin Map uploaded by the depositor. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have ...
Web16 feb. 2024 · mCherry-GAL9 is recruited to small molecule induced damage To validate that mCherry-GAL9 can be recruited to sites of endolysosome damage, we exposed … WebLive-cell mCherry-Gal9 assay with SSO-488 in HuH7 and HeLa cells a Live-cell confocal microscopy reveals a simultaneous decrease of SSO-containing bodies and Gal9 …
WebFigure Legend Snippet: a Overview of experimental approach. particles are formed by automated pipetting in a plate-based format and feed directly into plate-based assays for particle size determination by dynamic light scattering (DLS), encapsulation by ribogreen integration and for dosing mCherry-GAL9 reporter cells directly. b Heatmap summary of … Web1 apr. 2024 · A549 cells expressing FcγR2a and nuclear mCherry were cocultured with PBMCs in the presence of anti-CD3 antibody, Gal-9, and indicated control IgG or Gal-9 …
Web1 mrt. 2024 · mCherry-GAL9-recruitment assay. For experiments examining endosomal damage and mCherry-GAL9 recruitment, HeLa or HuH7_mCherry-Gal9 expressing cells were seeded into 384-well CellCarrier Ultra plates (PerkinElmer, cat no. 6007558) at 3000 or 3500 cells/well, respectively, 16 h before experimental usage.
WebmCherry-GAL9. Plasmid #166689. Purpose. For creating a stable mCherry-GAL9 cell line. Depositor. Alan Sabirsh. Article. ... Tags. mCherry . Expression. Mammalian . Mutation. … gifted intervention specialist endorsementWebGalectin-9 (GAL9) is a known marker of endosomal membrane disruption and can be utilized to quantitate endosomal rupture 22 . The initially selected 7 compounds and all … fry\u0027s 667Web1 mrt. 2024 · a HeLa_mCherry-Gal9 cells were treated with compound across 10-point dose titration series for 2 h and then stained for nuclei (Hoechst 33342, blue), fixed, and … fry\\u0027s 64th and greenwayWeb16 feb. 2024 · A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery. 1 Please help EMBL-EBI keep … fry\\u0027s 667WebmCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very … gifted intervention specialist programsWeb1 mrt. 2024 · Under normal conditions, mCherry-GAL9 is homogenously dispersed in the cytosol. When an endosomal leakage event is detected, mCherry-GAL9 will translocate … gifted instructor synonymWebGalectin-9 (GAL9) is a known marker of endosomal membrane disruption and can be utilized to quantitate endosomal rupture 22 . The initially selected 7 compounds and all … gifted italia