Hepg2 puromycin
Web10 jul. 2024 · HepG2-NTCP cells showed a constrained susceptibility, as described in a previous study 13. The differentiated HepaRG cells showed an infection pattern … Web11 apr. 2024 · VIP36-KO HepG2 cells were similarly transfected, and 24 h after transfection, the complexes were replaced with medium containing 2 μg/mL puromycin (#anti-pr-1, …
Hepg2 puromycin
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Web方法2：实验步骤大致为：预先对96孔板除第一排之外的所有孔加含15%胎牛血清的细胞培养液，0.1 ml/孔，并放于细胞培养箱中温育，然后胰酶消化稳定表达GFP的细胞，细胞液稀释至密度为1000 cell/ml的单细胞悬浮液，上述细胞液接种于96孔板的第一排，0.2 ml/孔，从中吸取0.1 ml细胞溶液接种于第二排，混合后，从第二排中吸取0.1 ml接种于第三排……， … WebThe generation of a stable cell line typically involves introducing a transgene or gene of interest into the genome of a host cell and selecting for cells that have integrated the …
Web2.1. Remove media containing lentiviral particle and add 120 µl of fresh media to each well. 2.2. Incubate 18-20 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7% CO 2. Day 3. 3.1. Aspirate virus-containing medium and add fresh media containing puromycin, as determined from antibiotic optimization. WebAfter 24 hours, the stably infected SNU-387 cell was selected with 2 μg/mL of Puromycin 2HCL (Selleck). The TMPO-AS1 expression vector (pcDNA3.1-TMPO-AS1, named as pc-TMPO-AS1) and empty pcDNA3.1 vector (negative control, named as pc-vector) ... Next, Huh7 and HepG2 cells were selected to construct TMPO-AS1-overexpressing cell lines.
WebPuromycin titration (kill curve) should be performed when working with a new cell type. 1. Plate 1.6 x 104 cells into wells of a 96 -well plate with 120 µl fresh media. 2. The next … Web应该使用哪种药物筛选标记？VectorBuilder提供了多种药物筛选标记，如嘌呤霉素（Puro），新霉素（Neo），潮霉素B（Hygro B）和杀稻瘟菌素（Bsd）。Puro比其他 …
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Web背景介绍. Luciferase Hep G2细胞稳定表达萤火虫荧光素酶。. 该细胞株性状稳定，培养时不需要添加抗生素维持。. 可用作萤火虫荧光素酶活性检测中的阳性对照，也可用于活体动物成像实验。. Hep G2细胞通过慢病毒转染的方式携带Luc基因。. Hep G2细胞是来自15岁男性 ... india richardsWeb7 uur geleden · Successfully transduced cells were selected with 10 µg ml −1 (293T) or 50 µg ml−1 (HepG2) puromycin (AdipoGen Life Science). ArgRS knockdown was confirmed via western blot and quantitative ... lockheed martin lighthouse centerWeb25 sep. 2024 · The HepG2 cells and HepG2-puro cells served as the naïve cell control and the transfection system control, respectively. (B) HBcAg synthesis in HBV-infected HepG2-SBP cells was detected by IFAs. HBcAg was stained with an anti-HBcAg mAb (red), and the nucleus was stained with DAPI (blue). lockheed martin lexington park mdWeb안녕하세요 바이오 고수님들께 궁금한 답을 찾고자 이렇게 글을 쓰게 되었습니다! 저희 실험실에서는 지금까지 CRI... lockheed martin life insuranceWebSCC064 Sigma-Aldrich LX-2 Human Hepatic Stellate Cell Line Download Zoom The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogen More>> india richest actor 2022WebSpandidos Publications india rice production by stateWeb13 apr. 2024 · DDX39B-deficiency HepG2 cells were cultured in complete medium containing D-glucose-13 C 6 (3.5 g/L) for 24 h. HepG2 cells were collected and lipids were extracted according to the protocols as previous described . Detection and metabolic flux analysis were performed by APExBIO Technology LLC. (Shanghai China). 2.14 … lockheed martin life insurance partners