WebIn the previous Glen Report, we introduced a new fluorescein phosphoramidite, 5’- dichloro-dimethoxy fluorescein, better known as JOE™ in the world of multicolor sequencing. This fluorescein derivative exhibits excellent stability to a range of deprotection conditions. In addition, its fluorescence is much less pH sensitive than fluorescein ... WebFor 5'-attachment, we have found 5'-Cholesterol-CE Phosphoramidite (LK2170) to offer specific advantages in oligo synthesis. Since the cholesterol is attached directly to aminohexanol, it is not susceptible to 1,2-diol elimination as observed in …
Fluorescein - Wikipedia
Webrequirements. 5'-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5'-hexachlorofluorescein-CE phosphoramidite (HEX) and 5' … WebDescription. 1-Ethynyl-dSpacer CE Phosphoramidite can be used in any position within an oligonucleotide while still retaining the high efficiency of click chemistry. The modifier is efficiently incorporated into oligonucleotides using standard phosphoramidite chemistry, is stable to common deprotection conditions, and is compatible with Glen ... kevin booth eversheds
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WebFluorescein-CE Phosphoramidite has a thiourea linkage which mimics the original method of incorporating fluorescein to an amino-modified oligo. It must be noted however that the linkage is attached via the 5 position of the ring system in this case. Internal sequence additions of Fluorescein are achieved using Fluorescein-dT. Web6-Fluorescein Phosphoramidite. Synonyms: 6-FAM-phosphoramidite. CAS Number: 204697-37-0. Molecular Weight: 843.94. Empirical Formula (Hill Notation): C 46 H 58 N 3 O 10 P. Product Number Product Description SDS; M041130: configured for ABI: Pricing: M041180: configured for PerkinElmer, configured for Polygen: WebStandard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports. Although 6-FAM, 6-HEX and 6-TET phosphoramidites have been effectively used for labeling oligos, there is no 6-JOE phosphoramidite available for preparing JOE-labeled oligos. is it worth to buy a house