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C11 bodipy flow cytometry

WebJul 1, 2013 · The probe C 11 -BODIPY 581/591 was used for measuring LPO in exposed algal cells (adapted from Cheloni and Slaveykova, 2013). ... Details on the flow … WebSS-treated SKOV3 cells for 24 h were incubated with C11-BODIPY 581/591, a lipid peroxidation sensor, for 15 min and analyzed using flow cytometry. Oxidized lipids were analyzed using FlowJo_v10.8.1 software. ( B ) Effect of carboplatin or paclitaxel on lipid peroxidation in SKOV3 cells.

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WebJun 9, 2024 · noic acid (C11-BODIPY581/591) to probe oxyl-radical induced lipid oxidation by flow cytometry (FCM). C11-BODIPY581/591 is a fatty acid analog with specific … WebFor lipid peroxidation evaluation, we used C11-BODIPY (BODIPY; D-3861; Molecular Probes) according to the protocol described by Guasti et al. (2012). BODIPY (5 μM) was added to the sample diluted according to a previous procedure and then incubated at 37°C for 30 min. ... Flow cytometry results (values expressed in percentage) at post-thaw ... fms ea toulouse https://obiram.com

Optimization of the C11-BODIPY(581/591) dye for the …

WebFluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C … WebSep 10, 2015 · • Label (Ex/Em): Bis-BODIPY™ FL C 11-PC (∼488/530 nm) • Specificity: PLA 1 or PLA 2 • Cleavage product: D3862 • Lyophilized product can be dissolved in ethanol for use • Fluorescence is measured either … WebNov 12, 2024 · The maximum emission wavelength of oxidized BODIPY shifted from 591 nm to 510 nm, which indirectly reflected the level of lipid peroxide. The lipid ROS levels were examined by employing the BD LSRFortessa Flow Cytometer (BD Biosciences, Franklin Lake, NJ, USA). 2.8. Malondialdehyde (MDA) Assay Utilizing an MDA assay kit (cat. greenshot filename pattern

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C11 bodipy flow cytometry

Comparison of the TBARS Assay and BODIPY C11 Probes for …

WebApr 14, 2024 · Indeed Bodipy 581/591 C11 staining of MPI KO and knock-down ... Representative plots of flow cytometry fluorescence intensity plot NT shRNA and MPI shRNA1 with AC220 (2.5 nM) ... WebBODIPY™ 581/591 undecanoic acid can be used to detect reactive oxygen species (ROS) in cells and membranes. Oxidation of the polyunsaturated butadienyl portion of the dye …

C11 bodipy flow cytometry

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WebApr 18, 2014 · The monitoring of lipid accumulation in microalgae is a key issue that needs an effective and fast method of analysis, mainly when biofuel-directed cultures are carried out. Boron-dipyrromethene (BODIPY), a green fluorescent lipophilic dye, was employed combined with flow cytometry for monitoring lipid storage in Nannochloropsis gaditana, … WebJan 1, 2009 · Flow cytometric two-dimensional dot-plots of fluorescence intensities of 10,000 individual boar sperm from thawed semen loaded with C 11-BODIPY 591/691 (BODIPY) and stained with propidium iodide. Sperm were incubated for 30 min at 37°C with a control treatment (no ROS generator) (panel a ) and with FeSO 4 -Na ascorbate …

WebJul 8, 2024 · We observed, by flow cytometry and fluorescence microscopy with BODIPY-C11 581/591 probe that the TMZ-resistant cell line T98G generates more lipid ROS when compared to U251MG cells (Fig. 2F–H). WebAug 13, 2013 · C11-BODIPY 591/581 staining was combined with flow cytometry measurements to obtain multiparameter information on …

WebThe measurement of peroxidation with BODIPY C(11) demonstrated significant dependence on the presence of a ferrous ion promoter (P < 0.001), which was significantly enhanced in sperm recovered from low-density Percoll fractions (P < 0.05) and was particularly damaging to the sperm midpiece. WebOct 14, 2024 · (D) MEF cells were treated with 1 μmol/L erastin for 8h, and flow cytometric detection of lipid ROS was performed using BODIPY C11. Representative data are shown and the statistical analysis is from three independent experiments.

WebMar 4, 2024 · Consistently, C11-Bodipy measurement indicated that cysteine deprivation increases lipid peroxidation in both cell lines, and deferoxamine could abrogate the effects of cysteine deprivation ( Fig. 1E and F ). Figure 1. View large Download slide Cysteine deprivation induces ferroptosis in HLRCC cells.

WebC 11 Bodipy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... red signifies cells incubated with C-11 BODIPY) using flow cytometry. Right: comparison of the percentages of C11-BODIPY-positive cells. … greenshot flouterWebAug 13, 2013 · C11-BODIPY(591/581) staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative stress damage within single cells. First, staining conditions were optimized by exploring the capability of the dye to stain algal cells under increasing cell and dye concentrations and … fms early yearsWebShift in Bodipy 581/591 C 11 fluorescence measured by flow cytometer. Bodipy 581/591 C 11 was excited using a 488 nm Ar laser and detected on a FL1 (515-545 nm) … fmse crackWebJul 1, 2013 · C11-BODIPY (591/581) staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative stress damage within single cells. greenshot for chromeWebAug 13, 2013 · C11-BODIPY(591/581) staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative … fmseattlegreenshot for linuxWebUse of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with 4, 4-difluoro-5- (4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid Authors H D Guthrie 1 , G R Welch Affiliation greenshot features