WebJul 1, 2013 · The probe C 11 -BODIPY 581/591 was used for measuring LPO in exposed algal cells (adapted from Cheloni and Slaveykova, 2013). ... Details on the flow … WebSS-treated SKOV3 cells for 24 h were incubated with C11-BODIPY 581/591, a lipid peroxidation sensor, for 15 min and analyzed using flow cytometry. Oxidized lipids were analyzed using FlowJo_v10.8.1 software. ( B ) Effect of carboplatin or paclitaxel on lipid peroxidation in SKOV3 cells.
Biomolecules Free Full-Text Gossypol Acetic Acid Attenuates …
WebJun 9, 2024 · noic acid (C11-BODIPY581/591) to probe oxyl-radical induced lipid oxidation by flow cytometry (FCM). C11-BODIPY581/591 is a fatty acid analog with specific … WebFor lipid peroxidation evaluation, we used C11-BODIPY (BODIPY; D-3861; Molecular Probes) according to the protocol described by Guasti et al. (2012). BODIPY (5 μM) was added to the sample diluted according to a previous procedure and then incubated at 37°C for 30 min. ... Flow cytometry results (values expressed in percentage) at post-thaw ... fms ea toulouse
Optimization of the C11-BODIPY(581/591) dye for the …
WebFluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C … WebSep 10, 2015 · • Label (Ex/Em): Bis-BODIPY™ FL C 11-PC (∼488/530 nm) • Specificity: PLA 1 or PLA 2 • Cleavage product: D3862 • Lyophilized product can be dissolved in ethanol for use • Fluorescence is measured either … WebNov 12, 2024 · The maximum emission wavelength of oxidized BODIPY shifted from 591 nm to 510 nm, which indirectly reflected the level of lipid peroxide. The lipid ROS levels were examined by employing the BD LSRFortessa Flow Cytometer (BD Biosciences, Franklin Lake, NJ, USA). 2.8. Malondialdehyde (MDA) Assay Utilizing an MDA assay kit (cat. greenshot filename pattern